A new common sense of stem cell culture born during the development of regenerative medicine

Serum-free AOF medium for mesenchymal stem cells

Image of serum-free AOF medium for mesenchymal stem cells Features of serum-free AOF medium for mesenchymal stem cells

*Stem cells cultured in Helene medium

Free of animal-derived ingredients	Reduced risk of infection from serum-derived components such as plasma
GMP compliant manufacturing	Fully comply with Japanese government regulations
Small difference between lots	Because there is little variation between lots, quality control of cell culture is possible.

Regenerative medicine product material qualification confirmation form

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DATA

Cell proliferation ability

Mesenchymal stem cells cultured in Helene Medium not only exhibit high proliferation potential but also maintain cell morphology.

cell surface marker

Mesenchymal stem cells cultured in Helene Medium exhibit a CD45 and CD105 profile.

After culturing adipose-derived hMSCs in Helene Medium, we analyzed the expression of cell surface markers by flow cytometry.

Three-way differentiation potential

Mesenchymal stem cells cultured in Helene Medium exhibit tridirectional differentiation potential into adipose, osteoblast, and fibroblast cells.

Adipose-derived hMSCs were cultured in Helene Medium and induced to differentiate into either adipose, osteoblast, or fibroblast cells. Cell differentiation was analyzed by histological staining.

Conference presentation materials

Presentation materials at the Cambridge International Stem Cell Symposium

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Born while developing regenerative medicine
New common sense in stem cell culture "Animal Origin Free"