ABOUT THE STEM CELL SUPERNATANT
About culture supernatant treatment

When stem cells are cultured, they
secrete components containing over 500 types of proteins.

These secretions
contain an abundance of cytokines, signaling substances that are key to cell activity, and

play an important role

in restoring the function of damaged tissues and cells within the body
This treatment is expected to have a variety of cosmetic effects as it promotes the recovery of cells that have weakened due to aging and other factors

Growth factors and effects

  • EGF epidermal growth factor

    1. Prevents and eliminates wrinkles by creating new skin cells
    2. get a healthy complexion
    3. Smooth and rejuvenate skin
    4. Accelerate wound healing by regenerating new skin cells
  • TGF-α transforming growth factor

    1. Increases skin elasticity by strengthening the structure of collagen and elastin
    2. Promotes skin growth and promotes wound healing
    3. Prevents and improves wrinkles by producing new cells
  • TGF-β transforming growth factor

    1. Prevent aging such as cell differentiation and induction
    2. Improves skin texture (involves collagen and elastin) and keeps skin youthful
    3. A factor that repairs without leaving a scar
  • PDGF platelet-derived growth factor

    1. Promote regeneration of damaged skin cells
    2. Promotes collagen synthesis and reduces wrinkles
    3. promote hair growth
  • VEGF vascular endothelial growth factor

    1. VEGF promotes hair growth by transporting nutrients to hair roots
    2. Prevents and improves wrinkles by producing new cells
    3. Gives energy to the skin and improves skin glow
  • IGF-I insulin-like growth factor

    1. Prevention and elimination of wrinkles by creating new skin cells
    2. Increase collagen, elastin and hyaluronic acid
    3. Improves skin feel and burns unwanted fat on the face and body
    4. Stimulates hair roots and strengthens hair

quality management


is carried out under strict control


throughout the entire process, from tissue receipt to cell culture and ensure high quality,

we have established an operational management system that incorporates a strict quality control system

  • Infectious disease and
    viral testing

  • Prepared using
    raw materials (culture media, etc.) that comply with biological raw material standards

  • Technology born
    from technological development for regenerative medicine products

At Helene Clinic,

we offer treatments
that utilize these safe

Click here for details of serum-free medium

Potential for culture supernatant treatment

Culture supernatant is the supernatant
obtained when stem cells present in the human body are cultured in a culture medium

The culture supernatant used for administration
is sterilized by filtering.

is rich in various cytokines

secreted by stem cells Cytokines
are proteins secreted by cells that
regulate cell proliferation and differentiation, and

play an


important role in restoring the functions of damaged tissues and cells within the body Cytokine-rich culture supernatant
promotes the recovery of cells weakened by aging and other factors, and
is therefore expected to have a variety of health and beauty benefits.


does not contain
any human- or animal-derived ingredients reducing the risk of infectious diseases.

stem cell extraction

Target therapist
  • 1) Those who have the ability to consent and can obtain consent from the person
  • 2) There is no age limit, but if the person is a minor, consent from a legal representative can be obtained.
  • 3) Those whose doctor has determined their eligibility based on a medical interview, examination, etc.
    (In principle, those with obvious serious, life-threatening illnesses are not eligible.)

In addition, there are selection criteria and exclusion criteria, so please contact the clinic for details.

duration of treatment For intravenous systemic administration (infusion): 40 to 60 minutes
Time required for cell administration 40 to 60 minutes
cost Intravenous systemic administration (infusion): 165,000 yen (tax included) *This treatment is not covered by public medical insurance.
Treatment risks and side effects Hearing loss, dizziness, tinnitus, kidney damage, hypoglycemia, infection, others
(anaphylactic reaction (cold sweat, nausea, vomiting, abdominal pain, difficulty breathing, low blood pressure),
pain at the puncture site, internal bleeding, nerve damage)

skin injury treatment

The culture supernatant is injected into areas where you want to see results. It is expected that cell division will become more active in the injected area, leading to tissue regeneration.
By injecting it subcutaneously into the scalp, it is expected to promote hair growth and improve the survival rate of hair transplants.

Intra-articular/injury pain relief

The culture supernatant is injected into the area where you want to see the effects. It is expected that cell division will become more active at the injected area, leading to tissue regeneration.
When injected into a joint, it is expected to suppress inflammation such as arthritis and regenerate the periosteum. In addition, various effects can be expected depending on the area.

Prevention of progression of arteriosclerosis and alleviation of chronic pain

The culture supernatant is added to a solution such as an intravenous drip and slowly administered intravenously.
Because the culture supernatant is rich in a wide variety of cytokines, it is expected to activate cells in damaged areas of the body and strongly restore various tissue functions from the aging phenomenon. Since
the insulin contained in the supernatant may cause hypoglycemia, you may be asked to ingest sugar to raise your blood sugar level before and after administration, and your blood sugar will be monitored with a simple blood glucose meter during administration.

If you are considering cell culture supernatant treatment,
please contact our clinic first.
We will provide you with detailed information and explanations about the treatment.

Click here for inquiries and counseling appointments

(1) Disclosure that it is an unapproved drug: This drug is not approved in Japan and will be prescribed at your own expense.
*Public relief systems (Drug Adverse Reaction Relief System, Biological Product Infection Relief System) do not apply to unapproved drugs.
(2) Disclosure of acquisition route, etc.: This drug is formulated in-house.
(3) Availability of domestically approved drugs with the same ingredients and efficacy: None.
(4) Disclosure of information regarding safety in other countries: Based on the safety standards for exosome therapy. No major adverse events have been reported.

FAQ

Q

How is the culture supernatant administered?

A

The administration method varies depending on the purpose of treatment.
Your doctor will suggest the most effective administration method, such as intravenous administration via drip infusion, local administration into subcutaneous tissue, muscles, or joints, or topical application.

Q

What is culture supernatant?

A

Culture supernatant is the supernatant obtained when stem cells present in the human body are cultured in a culture medium.
The culture supernatant used for administration has been sterilized by filtering.
Culture supernatant is rich in various cytokines secreted by stem cells. Cytokines
are proteins secreted by cells that regulate cell proliferation and differentiation, and play an important role in restoring the functions of damaged tissues and cells within the body
. Culture supernatant, rich in cytokines, promotes the recovery of cells weakened by aging and other factors, and
is therefore expected to have a variety of health and beauty benefits.

Q

What is the flow of culture supernatant treatment?

A

First, you will receive a consultation at the clinic, where we will explain the details of the treatment, costs, etc.
For a detailed explanation of the process after treatment, please see the page on the process from consultation to treatment (culture supernatant fluid treatment).

Click here for a list of frequently asked questions

Other treatment menu

stem cell therapy


This is a regenerative therapy in which
stem cells extracted from the patient are cultured and It is used to treat chronic pain and arteriosclerosis.

Other/Preventive treatment

We offer a variety of treatments, including those for preventing aging.

We provide various types of intravenous drips and cosmetic skin treatments

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Former Health, Labor and Welfare Minister Ritsuo Hosokawa
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