ABOUT THE STEM CELL SUPERNATANT
About culture supernatant treatment

When culturing stem cells, stem cells
It secretes components containing more than 500 kinds of proteins.

In secretions called cytokines
Rich in signaling substances that are the key to cell activity,
To restore function of damaged tissues and cells in the body
play an important role.

To support the recovery of cells that have weakened due to aging, etc.
It is a treatment method that is expected to have various effects on beauty.

Growth factors and effects

  • EGF epidermal growth factor

    1. Prevents and eliminates wrinkles by creating new skin cells
    2. get a healthy complexion
    3. Smooth and rejuvenate skin
    4. Accelerate wound healing by regenerating new skin cells
  • TGF-α transforming growth factor

    1. Increases skin elasticity by strengthening the structure of collagen and elastin
    2. Promotes skin growth and promotes wound healing
    3. Prevents and improves wrinkles by producing new cells
  • TGF-β transforming growth factor

    1. Prevent aging such as cell differentiation and induction
    2. Improves skin texture (involves collagen and elastin) and keeps skin youthful
    3. A factor that repairs without leaving a scar
  • PDGF platelet-derived growth factor

    1. Promote regeneration of damaged skin cells
    2. Promotes collagen synthesis and reduces wrinkles
    3. promote hair growth
  • VEGF vascular endothelial growth factor

    1. VEGF promotes hair growth by transporting nutrients to hair roots
    2. Prevents and improves wrinkles by producing new cells
    3. Gives energy to the skin and improves skin glow
  • IGF-I insulin-like growth factor

    1. Prevention and elimination of wrinkles by creating new skin cells
    2. Increase collagen, elastin and hyaluronic acid
    3. Improves skin feel and burns unwanted fat on the face and body
    4. Stimulates hair roots and strengthens hair

quality management

Culture supernatants are obtained from tissue receipts, cell cultures,
In a series of steps leading to quality control of the culture supernatant,
We operate under strict management.

In order to ensure high quality,
Operation management system that introduced a strict quality control system
building.

  • For patient cell culture
    by-products in
  • infections and
    Conducting virus tests
  • Based on biological raw material standards
    Compatible raw materials (medium, etc.)
    prepared using
  • For regenerative medicine products
    From technology development for
    born technology

At Helene Clinic
These safe
using culture supernatant
We provide treatment.

Click here for details of serum-free medium

Potential for culture supernatant treatment

Culture supernatant is a substance that exists in the human body.
Obtained when stem cells are cultured in a culture solution,
It will be the supernatant solution.
The culture supernatant used for administration is
Sterilized by filtering.
In the culture supernatant, secreted from stem cells
Rich in various cytokines.

Cytokines are
A protein secreted by cells
It has the function of regulating the proliferation and differentiation of cells,
of damaged tissues and cells in the body
important for functional recovery
playing a role.

The culture supernatant, which is rich in cytokines,
To support the recovery of cells that have weakened due to aging, etc.
You can expect various health and beauty effects.

The culture medium uses human and animal-derived components
I'm not here, so
It reduces the risk of infection.

stem cell extraction

Target therapist
  • 1) Those who have the ability to consent and can obtain consent from the person
  • 2) There is no age limit, but if the person is a minor, consent from a legal representative can be obtained.
  • 3) Those whose eligibility is recognized by the doctor in charge through interviews, tests, etc.
    (Clearly serious diseases that are life-threatening are not covered in principle.)

In addition, there are selection criteria and exclusion criteria, so please contact the clinic for details.

duration of treatmentFor intravenous systemic administration (infusion): 40 to 60 minutes 
Time required for cell administration40 to 60 minutes
costIntravenous systemic administration (infusion): 165,000 yen (tax included) *This treatment is not covered by public medical insurance.
Treatment risks and side effectsHearing loss, dizziness, tinnitus, kidney damage, hypoglycemia, infections, etc.
(Anaphylactic reaction <cold sweat, nausea, vomiting, abdominal pain, dyspnea, blood pressure drop>,
puncture site pain, internal bleeding, neuropathy)
 

skin injury treatment

Inject the culture supernatant by injection to the part where you want to get the effect. It is expected that cell division will be active at the injection site and tissue regeneration will occur.
Subcutaneous injection of the scalp is expected to increase the effect of hair growth and hair growth and increase the survival rate of hair transplantation.

 

Intra-articular/injury pain relief

Inject the culture supernatant by injection to the part where you want to get the effect. It is expected that cell division will be active at the injection site and tissue regeneration will occur.
When injected into the joint, it is expected to have the effect of suppressing inflammation such as arthritis and regenerating the periosteum. In addition, various effects can be expected depending on the part.

 

Prevention of progression of arteriosclerosis and alleviation of chronic pain

The culture supernatant is put into a solution such as an infusion and administered intravenously slowly.
Since the culture supernatant contains a wide variety of cytokines, it is expected to activate cells in damaged parts of the body and strongly restore various tissue functions from the aging process.
Insulin contained in the supernatant may cause hypoglycemic symptoms. We will check the status of

If you are considering culture supernatant treatment,
Please contact the clinic first.
We will guide you and explain the treatment in detail.

Click here for inquiries and counseling appointments

(1) Clarification that it is an unapproved drug: This drug is an unapproved drug in Japan and will be prescribed at your own expense.
   *Public relief systems (relief system for victims of adverse drug reactions, relief system for victims of biological product infections, etc.) are not applicable to unapproved drugs.
(2) Clarification of route of acquisition, etc.: This drug is an in-hospital preparation.
(3) Existence of domestically approved drugs with the same ingredients and efficacy: None
(4) Disclosure of information related to safety in other countries: Same as the safety of exosome therapy. No major adverse events were reported.

 

FAQ

 
Q

How is the culture supernatant administered?

 
A

The administration method differs depending on the purpose of treatment. Administer intravenously, subcutaneously or intramuscularly,
Your doctor will suggest the most effective administration method, such as topical administration to joints or application.

 
Q

What is culture supernatant?

 
A

Culture supernatant is the supernatant solution obtained when stem cells existing in the human body are cultured in a culture solution.
The culture supernatant used for administration is sterilized by filtration.
The culture supernatant is rich in various cytokines secreted by stem cells. Cytokines are secreted by cells
A protein that regulates the growth and differentiation of cells, and plays an important role in restoring the function of damaged tissues and cells in the body.
Plays. The culture supernatant, which is rich in cytokines, supports the recovery of cells that have weakened due to aging, etc.
You can expect various health and beauty effects.

 
Q

What is the flow of culture supernatant treatment?

 
A

First of all, you will receive a consultation at the clinic, and we will explain the details of the treatment and the cost.
For the detailed flow after treatment, please see the flow from counseling to treatment (culture supernatant treatment).

Click here for a list of frequently asked questions

Other treatment menu

stem cell therapy

Cultivating stem cells extracted from patients
It is a regenerative treatment that is put back into the body.
We treat chronic pain and arteriosclerosis.

Other/Preventive treatment

We will guide you through various treatments such as aging prevention.
Various drips, cosmetic skin treatment, etc.
This is done according to the patient's symptoms.

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culture video
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Former Health, Labor and Welfare Minister Ritsuo Hosokawa
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