In 2018, we presented the results of our self-made animal serum-free medium (HELENE Medium) at the Cambridge International Stem Cell Symposium. The first point is that more cells can be cultured with HELENE Medium than with other commercially available media in the same period of time. The second point is that the size of the mesenchymal stem cells cultured with HELENE Medium is the smallest. The academic evidence suggests that the smaller the stem cells are, the more unlikely they are to differentiate into fibroblasts. The above two points together confirm that the fat tissue taken from behind the ear does contain MSCs. The amount of fat collected is relatively low compared to that of the thigh or stomach, but with our own laboratory technology and HELENE Medium, it is possible to culture up to 2.25 billion MSCs in one month.

[Safe and pure stem cell culture technology and medium]
https://stemcells.jp/technique/