FAQ
Why is our hospital the only one that can provide 2.25 billion stem cell injections?
Our hospital's proprietary serum-free medium (HELENE Medium), vertical culture vessels, and experienced cell culture specialists - these three elements combined allow us to maintain the quality of the stem cells while culturing and administering up to 2.25 billion stem cells.
Without either of these, we would not be able to achieve this level of quality and quantity.
Where are stem cell products grown?
Stem cells for all patients at our hospital are consistently cultured in our in-house stem cell culture room (CPC).
All cell culture equipment used has received "Notification of Specific Cell Processing Product Manufacturing" from the Ministry of Health, Labor and Welfare, and has also obtained ISO 9001 certification (the AO Building branch is currently applying for certification).
About serum-free medium (HELENE Medium)
In 2018, we presented the results of our self-made animal serum-free medium (HELENE Medium) at the Cambridge International Stem Cell Symposium. The first point is that more cells can be cultured with HELENE Medium than with other commercially available media in the same period of time. The second point is that the size of the mesenchymal stem cells cultured with HELENE Medium is the smallest. The academic evidence suggests that the smaller the stem cells are, the more unlikely they are to differentiate into fibroblasts. The above two points together confirm that the fat tissue taken from behind the ear does contain MSCs. The amount of fat collected is relatively low compared to that of the thigh or stomach, but with our own laboratory technology and HELENE Medium, it is possible to culture up to 2.25 billion MSCs in one month.
[Safe and pure stem cell culture technology and medium]
https://stemcells.jp/technique/
How do you determine the number of stem cells?
After stem cell cultivation is complete, our hospital carries out the following three-step verification process.
First, we confirm that the target is a stem cell using the autoMACS magnetic bead separator.
The actual number of stem cells is then accurately determined using the Countess FL III automated cell counter.
In addition, we use an AI image recognition system developed in-house to evaluate the viability of stem cells.
Only cells that pass all three steps are handed over to the medical department for administration.
[About our stem cell culture room]
https://stemcells.jp/lab/
