FAQQ/A

FAQ

Q: Why is our hospital the only one that can provide 2.25 billion stem cell injections?

Our proprietary serum-free medium (HELENE Medium), vertical culture vessels, and experienced cell culture specialists - these three elements allow us to maintain the quality of the stem cells while culturing and administering up to 2.25 billion stem cells. Without any of these elements, we would not be able to achieve this level of quality and quantity.

Q: Where are stem cell products grown?

All of our patients' stem cells are cultured in our in-house stem cell culture room (CPC). All of our cell culture equipment has been approved by the Ministry of Health, Labor and Welfare as a "specific cell processing product manufacturing notification," and has also obtained ISO 9001 certification (the AO Building branch is currently applying for certification).

Q: About serum-free medium (HELENE Medium)

In 2018, we presented the results of our proprietary serum-free medium (HELENE Medium) at the Cambridge International Stem Cell Symposium. First, HELENE Medium allows for the cultivation of more cells in the same period than other commercially available media. Second, mesenchymal stem cells cultured in HELENE Medium were the smallest. There is scientific evidence that the smaller the stem cell, the less likely it is to differentiate into fibroblasts. Taking these two points into consideration, we confirmed that adipose tissue collected from behind the ear contains MSCs. Although the amount of fat collected is smaller than that from the thigh or abdomen, our proprietary laboratory technology and HELENE Medium enable the cultivation of up to 2.25 billion MSCs in one month. [Safe and Pure Stem Cell Culture Technology and Medium] https://stemcells.jp/technique/

Q: How do you determine the number of stem cells?

After stem cell cultivation is complete, our hospital carries out the following three-step verification process. First, we use an autoMACS magnetic bead separator to confirm that the subject is a stem cell. Next, we use a Countess FL III automated cell counter to accurately measure the actual number of stem cells. Furthermore, we use an AI image recognition system developed in-house to evaluate the viability of the stem cells. Only cells that pass all three steps are handed over to the medical department and used for administration procedures. [About our stem cell culture lab] https://stemcells.jp/lab/

All related to stem cells, procedures , treatment efficacy, safety, culture technology, and laws/approvals.

Why is our hospital the only one that can provide 2.25 billion stem cell injections?

Our hospital's proprietary serum-free culture medium (HELENE Medium), combined with vertical culture vessels and experienced cell culture specialists, allows us to maintain the quality of stem cells while culturing and administering up to 2.25 billion stem cells. Without
any of these elements, this level of quality and quantity cannot be achieved.

Where are stem cell products grown?

All of our patients' stem cells are consistently cultured in our in-house stem cell culture laboratory (CPC).
All cell culture equipment used has received approval from the Ministry of Health, Labour and Welfare for "Specific Cell Processing Product Manufacturing Notification," and is also ISO 9001 certified (application for ISO 9001 certification is currently pending at our AO Building branch).

About serum-free medium (HELENE Medium)

In 2018, we presented the results of our self-made animal serum-free medium (HELENE Medium) at the Cambridge International Stem Cell Symposium. The first point is that more cells can be cultured with HELENE Medium than with other commercially available media in the same period of time. The second point is that the size of the mesenchymal stem cells cultured with HELENE Medium is the smallest. The academic evidence suggests that the smaller the stem cells are, the more unlikely they are to differentiate into fibroblasts. The above two points together confirm that the fat tissue taken from behind the ear does contain MSCs. The amount of fat collected is relatively low compared to that of the thigh or stomach, but with our own laboratory technology and HELENE Medium, it is possible to culture up to 2.25 billion MSCs in one month.

[Safe and pure stem cell culture technology and culture medium]
https://stemcells.jp/technique/

How do you determine the number of stem cells?

At our hospital, after the completion of stem cell culture, we perform the following three-stage verification process.
First, we use an autoMACS magnetic bead separator to confirm that the cells are indeed stem cells.
Next, we accurately measure the actual number of stem cells using a Countess FL III automated cell counter.
Furthermore, we evaluate the viability of the stem cells using an AI image recognition system developed in-house.
Only cells that clear all three of these steps are handed over to the medical department and used for administration.

[About our stem cell culture laboratory]
https://stemcells.jp/lab/